The Effect of Using Bone Marrow Mesenchymal Stem Cells Versus Platelet Rich Plasma on the Healing of Induced Oral Ulcer in Albino Rats

BACKGROUND AND OBJECTIVES: Oral ulceration is one of the most common debilitating condition that affects the oral cavity. In this study, the effect of locally injected platelet rich plasma (PRP) and bone marrow-derived mesenchymal stem cells (BMSCs) on the healing of oral ulcer was investigated. METHODS AND RESULTS: An ulcer was induced in buccal mucosa of rats by using 5mm biopsy punch followed by application of cotton swab soaked with formocresol for 60sec. The ulcer was left untreated in the control group, treated with intralesional injection of PRP, or isolated cultured BMSCs. Data were analyzed clinically, histologically and immunohistologically on day 3, 5, 7 and 10. BMSCs group showed smaller ulcer area throughout the whole experimental period than the other groups with complete resolution of the ulcer on day 10, unlike the control group. However, there was no significant difference with PRP, on day 5, 7 and 10, regarding clinical ulcer size. BMSCs group showed better histological results regarding the rate of epithelial cell migration, the number of inflammatory cells, thickness and organization of collagen fibres and the number of blood vessels, with complete re-epithelization on day 10. BMSCs group showed a greater number of anti-PCNA positive nuclei throughout the whole experimental period than the other groups except on day 5, PRP had higher mean numbers of anti-PCNA positive nuclei in both tissues. CONCLUSIONS: Both PRP and BMSCs accelerate wound healing and enhance the quality of the healing tissue with the latter being slightly more effective and faster.

Influence of sympathetic activity on the progression of chronic kidney disease

There is clear evidence that chronic kidney disease [CKD] is often characterized by the presence of sympathetic hyperactivity. Data accumulating that this sympathetic hyperactivity is indeed important, because it may influence cardiovascular and renal prognosis. The aim of this study was to assess the relationship between glomerular filtration rate [GFR] and the levels of norepinephrine [NE] in serum and urine in the presence of variable degrees of renal functional impairment. A total of 75 CKD patients were divided into 5 groups according to GFR, group 1: 15 CKD patients with GFR>90ml/min/1.73m2 [stage 1CKD]. group 2: 15 CKD patients with GFR 60-89ml/min/1.73m2 [stage 2 CKD]. group 3: 15 CKD patients with GFR 30-59ml/min/1.73m2 [stage 3 CKD]. group 4: 15 CKD patients with GFR 15-29ml/min/1.73m2[stage 4 CKD]. group 5: 15 CKD patients with GFR<15ml/min/1.73m2 [stage 5, endstage renal failure], in addition to 15 healthy controls were studied. GFR was estimated by Cockroft-Gault formula. Norepinephrine was measured by an enzyme-linked immunosorbent assay. In addition, blood urea, serum creatinine, C-reactive protein [C-RP], fasting blood sugar [PBS], serum total cholesterol, triglycerides and 24-h urinary proteins were performed. Compared with controls, CKD patients had higher levels of serum norepinephrine, urinary norepinephrine was significantly lower among CKD patients. When GFR was reduced in CKD patients, serum norepinephrine was elevated and urinary norepinephrine was reduced suggesting greater renal impairment. In multivariate correlation, GFR were negatively correlated with serum norepinephrine and positively correlated with urinary norepinephrine. Serum norepinephrine levels were increased and urinary norepinephrine excretion were decreased in CKD patients, and may be one of the aggrevating factors for deterioration of renal function in those patients

Apoptosis suppressing protein Bcl-2 expression in rheumatoid synovium

It has been suggested that defective apoptosis plays a role in the development of autoimmune. The aim of this study is to investigate the apoptosis suppressing protein Bcl-2 in the rheumatoid synovum. Twenty synovium biopsy specimens were studied histopathologically and immuno-histochemically. Positive immunoreactivity was observed in 16 cases [80%] the infiltrated lymphocytes and in 11 cases [55%] in the lymphoid follicles, while, in 8 cases [40%] deep synovial fibroblasts showed marked Bcl-2 positively. The superficial synovial cell lining was only positive in 5 cases [25%].The apoptotic process is suppressed in rheumatoid arthritis as triggered by Bcl-2 oncoprotein. The defective control of apoptosis. [programmed cell death] as well excessive proliferation may he of central importance in the pathogenesis of rheumatoid arthritis

Radiofrequency palatal reduction versus LAUP as a treatment of snoring and mild obstructive sleep apnea

In this study, 30 patients complaining of snoring and mild obstructive sleep apnea were randomly distributed into two groups, 15 patients each. One group was subjected to laser assisted uvulopalatoplasty [LAUP] while the other to RF. The follow up of these patients revealed that LAUP is more effective than RF, whereas RF is more tolerable than LAUP. LAUP was proved to be an effective method in the treatment of snoring and mild obstructive sleep apnea, while RF proved to be very safe and well-tolerated but needs further study to exactly evaluate its efficacy